Wang CY, Lin MH, Su HT (2016) A method for measuring RNA N 6-methyladenosine modifications in cells and tissues. We confirmed that INB with the antibodies for 1-methyladenosine (m1A), N6-methyladenosine (m6A), pseudouridine, and 5-methylcytidine (m5C) showed different modifications in a variety of RNAs from.Feederle R, Schepers A (2017) Antibodies specific for nucleic acid modifications.Yamasaki S, Ivanov P, Hu GF, Anderson P (2009) Angiogenin cleaves tRNA and promotes stress-induced translational repression.Mishima E, Inoue C, Saigusa D, Inoue R, Ito K, Suzuki Y, Jinno D, Tsukui Y, Akamatsu Y, Araki M, Araki K, Shimizu R, Shinke H, Suzuki T, Takeuchi Y, Shima H, Akiyama Y, Toyohara T, Suzuki C, Saiki Y, Tominaga T, Miyagi S, Kawagisihi N, Soga T, Ohkubo T, Yamamura K, Imai Y, Masuda S, Sabbisetti V, Ichimura T, Mount DB, Bonventre JV, Ito S, Tomioka Y, Itoh K, Abe T (2014) Conformational change in transfer RNA is an early indicator of acute cellular damage.When the rabbit antiserum was used in a western blot of human soluble proteins, it detected the 25-kDa protein, but also bound to proteins of 65 kDa and 39. Mishima E, Jinno D, Akiyama Y, Itoh K, Nankumo S, Shima H, Kikuchi K, Takeuchi Y, Elkordy A, Suzuki T, Niizuma K, Ito S, Tomioka Y, Abe T (2015) Immuno-northern blotting: detection of RNA modifications by using antibodies against modified nucleosides. Assuming that a pure protein was used to make the antibodies, how do you explain the results of the western blot Antibodies were produced in a rabbit against a 25 kDa human soluble protein.Stable Markers for Northern Analysis - TechNotes 8(4)ĭescription: Markers provided in formamide for increased RNase resistance. Premade, RNase-free Northern Reagents - TechNotes 9(1)ĭescription: Increase Northern sensitivity by limiting degradation of probe and of RNA samples bound to your blots Northern Analysis: Faster and Better - TechNotes 11(2)ĭescription: Decrease hybridization and exposure time with ULTRAhyb® Ultrasensitive Hybridization Buffer. Get More From Your Blots with ULTRAhyb® Technology - TechNotes 14(2)ĭescription: Here we show that by selecting a more sensitive hybridization buffer, the percentage of target molecules that hybridize to probe on a Northern blot can be significantly increased. WB is simple, unique, rapid, widely used routine tool with easy interpretation and definite results. Ten Ways to Increase the Sensitivity of Northern Hybridizationsĭescription: Reduce Northern blot exposure times from days to hours by pushing blot hybridizations towards completion Western blotting (WB), also known as immunoblotting, is a well-known molecular biology method that biologists often use to investigate many features of the protein, ranging from basic protein analysis to disease detection. If you have never performed Northern analysis, or if you want to optimize your Northern blot experiments, you will definitely want to check out this informative article. Western blot is used for a) DNA analysis b) RNA analysis c) protein. Northern blot is used for a) DNA analysis b) RNA analysis c) protein an 6. DNA analysis b) RNA analysis c) protein analysis 5. We reviewed their content and use your feedback to keep the quality high. All of these can be used to confirm results from microarray analysis and differential display experiments.Ĭomparison of Different Methods of mRNA Quantitationĭescription: A brief comparison of nuclease protection assays, Northern blotting, RT-PCR and in situ hybridization for the detection and quantitation of RNA.ĭescription: "Northern Analysis: The Basics" leads you through the steps involved in Northern blotting and explains the considerable improvements Ambion has found over standard Northern blotting protocols. In the northern blot, another variation of the Southern blot, RNA (not DNA) is immobilized on the membrane and probed. Experts are tested by Chegg as specialists in their subject area. Includes recommendations for both radiolabeled and nonisotopically labeled DNA, RNA and oligonucleotide probes.Īnalysis of Differential Gene Expression - Technical Bulletin #500ĭescription: Here, we review three popular RNA detection/quantitation methods: Northern blot analysis, Ribonuclease Protection Assays (RPAs) and Reverse Transcription Polymerase Chain Reaction (RT-PCR). Amount of Probe to Use in a Northern Blotĭescription: A quick chart of various probe concentrations to use in your Northern blotting experiments.
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